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Pnxgate32-3ha

WebSep 23, 2010 · Members of the HMT-1 (heavy metal tolerance factor 1) subfamily of the ATP-binding cassette (ABC) transporter superfamily detoxify heavy metals and have unique topology: they are half-molecule ABC... WebPNXGATE32-3HA : plasmid : High copy Split Ubiquitin vector for fusing proteins with the NubG peptide. The resulting fusions contain NubG at the N-terminus and a tripple HA tag …

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WebOct 19, 2010 · Showing the operation of my Nathan Airchime K3HA Train Horn. I ran this on my car with a hornblasters air tank and pump kit. WebObrdlik P, El Bakkoury M, Hamacher T, Cappellaro C, Vilarino C, Fleischer C, et al.K+ channel interactions detected by a genetic system optimized for systematic studies of membrane protein interactions. tango smrti majica https://tanybiz.com

Detecting interactions with membrane proteins using a

WebMar 29, 2024 · The CDS of OsPLGG1b was cloned into pNXgate32-3HA to produce Nub-OsPLGG1b prey plasmids. The bait and prey vectors were transformed into the yeast … WebpMetYCgate, pNXgate32-3HA have been described in detail (Grefen et al., 2007). The vector pMetYCgate is used to create C-terminal Cub fusion proteins and the vector pNXgate32 … batam shipyard

Name / Stock Number: pNXgate32-3HA / CD3-935

Category:实验技能丨酵母双杂交实验超详细protocol - 知乎

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Pnxgate32-3ha

Manual for the use of mating-based Split ubiquitin system "mbSUS …

WebUSB ports on the device can be used for a variety of purposes. The primary use for the USB ports is to install or reinstall the operating system on the device. Beyond that, there are … WebThe SG-3100 has two slots capable of installing M.2 SATA drives, J10 and J11. The J10 connector is for a 2280 (22mm x 80mm) M.2 SATA only. The 80mm standoff cannot be …

Pnxgate32-3ha

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WebDownload Publication - Boyce Thompson Institute - Cornell University Web实验步骤. 酵母双杂交系统正是利用了GAL4的功能特点,通过两个杂交蛋白在酵母细胞中的相互结合及对报告基因的转录激活来捕获新的蛋白质,其大致步骤为: 1、 构建重组Activation Domain(AD)融合的重组质粒(pGADT7载体)以及Binding Domain(BD)融合的重组质粒 …

WebZestimate® Home Value: $831,400. 3 Northgate Dr, Ponte Vedra Beach, FL is a single family home that contains 2,037 sq ft and was built in 1983. It contains 2 bedrooms and 2 … WebDescription: Control construct for split ubiquitin system in pNXgate32-3HA vector. Growth Requirement: grow in LB media at 37C for 12 to 24 hours Marker: ampicillin Background: ABRC Comment: Format Shipped: bacterial stab Base / Commercial Price: $13 / $104

WebObrdlik P, El Bakkoury M, Hamacher T, Cappellaro C, Vilarino C, Fleischer C, et al.K+ channel interactions detected by a genetic system optimized for systematic studies of membrane … WebSep 23, 2010 · Members of the HMT-1 (heavy metal tolerance factor 1) subfamily of the ATP-binding cassette (ABC) transporter superfamily detoxify heavy metals and have unique topology: they are half-molecule ABC transporters that, in addition to a single transmembrane domain (TMD1) and a single nucleotide-binding domain (NBD1), possess …

WebThe cDNA library was cloned in the multi-copy prey plasmid pNXgate32 3HA (Obrdlik et al., 2004). Before shuttling of the cDNA library, the ccdB gene was inserted into the prey …

WebDec 10, 2016 · The full-length HSP21 protein fused to the NubG fragment of the pNXgate32-3HA vector was used as the prey. The full-length mature subunits of D1, D2, CP47, PsaA and PsaB fused to the LexA-VP16-Cub fragment of the pNCW vector were used as bait. batam sea portWeb(i) The pNXgate33-3HA is a low-copy CEN plasmid, which mediates significantly lower expression of NubG-ORF fusions when compared to pNXgate32-3HA. Therefore the use … tango snack produkWebA yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells tango sneek prijsWebAssociomics split-ubiquitin system expression clonein pNXgate32-3HA vector, can be used to screen for protein-protein interactions using a mating based split-ubiquitin system screen. Growth Requirement: grow in LB media at 37 C overnight. Marker: … tango tv-kanavaWebThis website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies. batam singapore borderWebJan 31, 2007 · Description: High copy Split Ubiquitin vector for fusing proteins with the NubG peptide. The resulting fusions contain NubG at the N-terminus and a tripple HA tag at the … tango strojWebpNXgate32-3HA was a gift from Wolf Frommer (Addgene plasmid # 105076 ; http://n2t.net/addgene:105076 ; RRID:Addgene_105076) For your References section: K+ … pNXgate32-3HA Sequences (1) Addgene Sequences: Full (1) Full Sequences from … batam software digital