Web19 iul. 2016 · In addition to degradation, recent studies have revealed the roles of lysosomes in metabolic pathways through kinases such as mechanistic target of rapamycin (mTOR) and transcriptional regulation through calcium signaling molecules such as transcription factor EB (TFEB) and calcineurin. Web2 aug. 2024 · For vacuolar patch clamp measurements, cells were treated with apilimod (1 µM) for 14 h to 18 h to enlarge endo-lysosomal organelles. For plate reading, cells were plated onto opaque-walled 96 ...
Lysosome electrophysiology - PubMed
Web11 dec. 2024 · Patch-clamp studies of isolated lysosomal membranes have recently discovered multiple lysosomal channels that are selective for Na +, K +, Ca 2+, and Cl … Web1 aug. 2024 · Here we present a protocol detailing how to implement a manual patch-clamp technique for endolysosomal compartments. In contrast to the alternatively used planar endolysosomal patch-clamp... chelsea95
Regulation of lysosomal ion homeostasis by channels and
WebThe patch-clamp technique is an electrophysiological method that allows the recording of whole-cell or single-channel currents flowing across biological membranes through ion channels. Using patch clamp we have the possibility to control and manipulate the voltage (voltage clamp) of membrane patches or whole cells such as hair cells. Web14 iun. 2024 · A deep-learning-based tool and a large ground truth dataset enable spike inference from calcium imaging data… Nina Vogt Article 14 June 2024 Metabolomic … Web30 apr. 2024 · containing lysosomes was resuspended in 40 l of washing buffer, which was used for whole lysosome patch-clamp recording. All steps were performed on ice to minimize the activation of damaging from intracellular phospholipases and proteases. Lysosomes were used for electrophysiological recordings within3hofisolation to keep … chelsea945