Lactobacillus species by pcr
WebNov 23, 2024 · The standard curves for qPCR of the three Lactobacillus species were successfully established and applied to monitor the changes in their populations during … WebDec 8, 2015 · Methods and Results. A total of 470 LAB were initially isolated from 53 healthy individual and characterized to species level. Of these, 88 (86%) were Lactobacillus species. Biochemical and genetic fingerprinting with Phene-Plate system (PhP-LB) and RAPD-PCR showed that the isolates were highly diverse consisted of 67(76.1%) and 75 (85.2%) single …
Lactobacillus species by pcr
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WebThis method was applied to a collection of 82 Lactobacillus strains belonging to 37 species in order to evaluate the discriminatory power of this technique within this genus. Twenty-one species could be distinguished to species level on the basis of a … WebMay 11, 2024 · Quantitative PCR of Lactobacillus species isolated through culture revealed no statistically significant difference between the epileptic and control dogs (median concentration, 3.8 log10 CFU/g feces and 4.6 log10 CFU/g feces, respectively). Lactobacillus in culture was not killed by exposure to phenobarbital, potassium bromide, zonisamide, or ...
WebApr 15, 2024 · In this study, we developed specific primer pairs using comparative genomics to identify Lactobacillus accurately and rapidly at the species level, then applied this technology in the PCR assays that can detect and identify 37 Lactobacillus species in a … WebJan 17, 2014 · The 279 Lactobacillus signals detected by PCR-DGGE technique belonged to 13 different species. Lactobacilli of the L. delbrueckii group ( L. gasseri , L. crispatus , L. …
WebSep 1, 2024 · The Lactobacillus casei group species are used in dairy fermentation and can improve the flavor and texture of food products. They produce many bioactive … WebJan 17, 2014 · The 279 Lactobacillus signals detected by PCR-DGGE technique belonged to 13 different species. Lactobacilli of the L. delbrueckii group ( L. gasseri , L. crispatus , L. johnsonii, L. iners, L ...
WebAug 28, 2024 · Equipment and Software. Anvi’o, Bacterial Pan Genome Analysis pipeline (BPGA), USEARCH, and Basic Local Alignment Search Tool (BLAST) software were used for comparative genomics to select specific genetic genes for Bifidobacterium species or subspecies. 7500 Real-time PCR System (Applied Biosystems, Foster City, CA, United …
WebIn the present study, we developed a novel real-time PCR assay for distinguishing the L. casei group species. The pan-genome, as determined by the genomes of 44 strains, … inglewood customs eastman gaWebMar 20, 2024 · Many Lactobacillus species are frequently isolated from dairy products, animal guts, and the vaginas of healthy women. However, sequencing-based identification of isolated Lactobacillus strain is time/cost-consuming and lobor-intensive. In this study, we developed a multiplex PCR method to distinguish six closely related species in the … inglewood country club membership costWebReal-time quantitative PCR was performed using genus and species-specific primer sets ( Table 1 ), to measure the quantity of the total Lactobacillus and the quantity of each of the seven Lactobacillus species detected by conventional PCR in each fecal sample. inglewood city council membersWebApr 1, 2024 · Numerous Lactobacillus species have been detected in the vaginal milieu, among which the following four species typically dominate: L. crispatus, L. jensenii, L. gasseri and L. iners [1], [2]. Genus-, group- or species-specific PCR assays are used for vaginal lactobacilli detection. inglewood continuation high schoolWebAug 1, 2002 · In this study, species-specific PCR and HRM (high-resolution melting) analysis were developed to identify strains belonging to the Lactobacillus casei group and to classify them into L. casei, Lactobacillus paracasei and Lactobacillus rhamnosus. HRM analysis confirmed to be a potent, simple, fast and economic tool for microbial identification. inglewood courthouse addressWebDec 19, 2007 · Background Most studies of the vaginal microflora have been based on culture or on qualitative molecular techniques. Here we applied existing real-time PCR formats for Lactobacillus crispatus, L. gasseri and Gardnerella vaginalis and developed new formats for Atopobium vaginae, L. iners and L. jensenii to obtain a quantitative non culture … inglewood courthouse casesWebSpecies-specific quantification of probiotic lactobacilli in yoghurt by quantitative real-time PCR The real-time PCR assay developed here might become a convenient tool enabling an accurate, fast and sensitive detection of probiotic lactobacilli commercially used in food. inglewood courthouse jobs