WebBuffer composition Buffer Content HBS-N Aqueous buffer containing 0.01 M HEPES pH 7.4, 0.15 M NaCl HBS-P As HBS-N, with 0.005% (v/v) Surfactant P20 HBS-EP As HBS … WebFeb 15, 2024 · Antibodies were 2-fold serially diluted from 1.250 to 0.039 μg/mL in HBS-EP buffer (Cytiva) and then injected for 120 s at 30 μL/min. After that, the binding was dissociated with HBS-EP buffer ...
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WebAug 31, 2024 · HBS-EP running buffer was used to prepare the samples, then injected onto the functionalized surface. A 1 min pulse of 10 mM glycine pH 3.0 (Cytiva Co., Ltd., Shinjuku, Tokyo, Japan) was used to perform regeneration at the end of each kinetic cycle. The HBS-EP solution was chosen as the control. WebApr 8, 2024 · Different concentrations of RBD (250–1000 nM), from the Bates Lab, was diluted using HBS-EP + buffer and injected over the sensor chip at a flow rate of 30 µL/min for 3 min as an association phase.
Webcytiva.com 29396778 AC. Additional materials required: • Sensor Chip CM5 (BR100399, BR100012, or 29149604). • HBS-EP+ 10× buffer (BR100826 or BR100669). Dilute 1+9 (v/v) with deionized, 0.2 μm filtered water to make the running ... Do not use running buffer with additives containing metal ions WebA Biacore X-100 system with the Biotin CAPture kit (cytiva) and the HBS-EP + running buffer (cytiva) were used. The RBD of SARS-CoV-2 was purchased with an AviTag (Acrobiosystems) and captured to a level of 100 RU. Increasing concentrations of the ACE2-IgG4-Fc (0.32, 1.6, 8, 40 and 200 nM) were injected over the immobilized RBD in a …
Webweight 26 kDa), 0.2 mg/ml in HBS-EP buffer (10 mM HEPES pH 7.4, 0.15 M NaCl, 3 mM EDTA, and 0.005% Surfactant ... cytiva.com 22052219 AI. Antibody information Anti-GST antibody is a polyclonal goat antibody recognizing GST. Required materials See the list below for additional required materials (available from Cytiva). WebApr 1, 2024 · Interactions between VWF A1 and the anti-Flag captured GPIbα were measured in HBS-EP buffer (Cytiva Life Sciences) at a flow rate of 30 µL/min. Each binding cycle began with loading 3 µM WT or p.Arg127Gln GPIbα, followed by a 100-second dissociation phase. Then VWF A1 was allowed to bind for 100 seconds, followed by a …
WebTechnical Specs. Application: Analytical chemistry. Research. For most types of interaction analyses in Biacore systems. For use with: For use in Biacore C, and Biacore 3000 SPR …
WebHBS-EP+ Buffer 10×. General purpose running buffer. Concentrated stock solution yielding pH 7.4 when diluted 10x. General purpose running buffer. Concentrated stock solution … chemical induction systemWebOct 8, 2024 · Surface plasmon resonance (SPR) measurements were performed at 25 °C, on Biacore ® 3000 instrument (Wipro GE Healthcare Pvt. Ltd.), using HBS-EP + running buffer (Cytiva Sweden AB, Cat. No. BR100669). Fc fragment specific, goat polyclonal anti-human IgG (Jackson Immuno Research, Cat. No. 109-001-008) in 10 mM sodium … chemical induction spray3rWebHBS-EP+ Buffer 10×. General purpose running buffer. Concentrated stock solution yielding pH 7.4 when diluted 10x. Order Product flight as005WebApr 21, 2024 · Purified ACE2 in HBS-EP buffer (Cytiva) at 300 nM was loaded on an individual biosensor followed by a 1 min wash in HBS-EP buffer. Afterward, a 10 min association was performed in the sample plate with either 150 nM RBD or RBD combined with mAbs. The results were analyzed using Data Analysis Software 7.0 (Pall ForteBio … flight as008WebCytiva HBS-EP Buffer, 1000mL, 10X Concentration, 7 4 pH, Colorless, Liquid, Stored at Room Temperature, Contents 0 1M HEPES, 1 5M NaCl, 0 03M EDTA and 0 5% v v … flight as0111WebMar 10, 2024 · For affinity analysis, the spike protein (S1 + S2), S1 subunit or S2 subunit were dissolved in HBS-EP + running buffer at concentrations of 78.125, 156.25, 312.5, 625, and 1250 nM and were run ... chemical inductor coneWebRequired buffer Running buffer e.g.,HBS-EP: 10 mM HEPES pH 7.4, 0.15 M NaCl, 3 mM EDTA, 0.05% Surfactant P20, HBS-P, HBS-N, PBS Important Note Start-up cycles: For best assay performance, run at least one start-up cycle using identical settings as for the analysis cycles, including Twin-Strep-tag® fusion chemical inducer of dimerization