WebCells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor ® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies. WebSecondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make them useful for detection, purification, or sorting applications. We offer secondary antibodies from a variety of host species.
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WebBlue Prestained Protein Marker, Broad Range (11-250 kDa): . Blotting Membrane and Paper: This protocol has been optimized for nitrocellulose membranes. Pore size 0.2 µm is generally recommended. Secondary Antibody Conjugated to HRP: Anti-rabbit IgG, HRP-linked Antibody . Detection Reagent: SignalFire™ ECL Reagent . WebThis product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free … la county library renewal
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WebApr 14, 2024 · Secondary only controls were prepared by omitting the primary Anti-beta2-adrenergic receptor Ab staining step. The analysis of the stained samples was done using a BD FACSCantoTM II flow cytometer and computer equipped with the BD FACSDivaTM software (BD Biosciences, version 6.1.3). Data was analysed by the FlowJo software … WebSave time and replace your secondary antibodies with our FlexAble Antibody Labeling Kits $389 / 150 μL Cat No. 21829-1-AP In stock. Order within 62 hours and 35 mins to receive by Tuesday, March 21 Freight/Packing: $40.00 Country/Region Size/Concentration Quantity Add to Basket Proteintech Guarantee Request bulk or custom quote WebSecondary antibodies indirectly detect a primary antibody that specifically binds to the protein of interest. They can be conjugated to fluorophores (eg Alexa Fluor® dyes … la county library paramount